FIGURE

Fig. 2

ID

ZDB-FIG-180613-20

Publication

Kasheta et al., 2017 - Identification and characterization of T reg-like cells in zebrafish

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Fig. 2

Identification and analysis of foxp3a:EGFP-positive thymocytes. (A) Flow cytometry analysis of thymus from representative AB and Tg(foxp3a:EGFP) animals. (B) qRT-PCR of selected genes in foxp3a:EGFP-positive thymocytes relative to bulk thymocytes. Relative CD4 and CD8 expression is also shown and represents zebrafish cd4-1 and cd8a genes. ?, Expression of cd8a was below the limit of detection in foxp3a:EGFP-positive thymocytes. Error bars indicate SEM.; n = 3. (C) Cells from dissected thymus of a Tg(foxp3a:EGFP); Tg(rag2:mCherry) animal showing a double-positive thymocyte (arrowheads). Bars, 10 µm.

Expression Data

Gene:	EGFP
Fish:	zf2131Tg
Anatomical Term:	pro-T cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Med.