FIGURE

Fig. 1

ID

ZDB-FIG-180608-88

Publication

Paone et al., 2018 - Loss of zebrafish Smyd1a interferes with myofibrillar integrity without triggering the misfolded myosin response

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Fig. 1

Smyd1a is specifically expressed in the heart and fast-twitch skeletal muscle cells. (A, B) smyd1a mRNA is strongly expressed in somatic muscle cells and both heart chambers (inset) as revealed by ISH. (B) Smyd1 specific RT-PCR confirms expression of all smyd1 transcription variants in embryonic heart tissue. (C) Smyd1a-specific ISH and immunostaining of slow-twitch skeletal muscle fibers (slow MHC) in a transversal section of a zebrafish embryo. (D) Fluorescent imaging of transgenic lines carrying either a smyd1b_tv1-gfp fusion construct or smyd1a-gfp fusion construct under the muscle specific unc45b^min-promoter in combination with co-immunostaining against sarcomeric α-Actinin (Z-disc marker).

Expression Data

Genes:	EGFP smyd1a smyd1b
Fish:	WT ulm100Tg ulm102Tg
Anatomical Terms:	fast muscle cell heart skeletal muscle M band
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.