FIGURE

Fig. 3

ID

ZDB-FIG-180608-90

Publication

Paone et al., 2018 - Loss of zebrafish Smyd1a interferes with myofibrillar integrity without triggering the misfolded myosin response

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Fig. 3

Smyd1a is required for proper sarcomere organization and can compensate for the loss of Smyd1b. (A) Co-immunostaining of plasmid (unc45b^min:smyd1b_tv1-gfp- or with unc45b^min:smyd1a-gfp) injected fla mutants with sarcomeric α-Actinin. [Scale bar: 20 μm]. (B) Flatline embryos were injected with either KCl (control), smyd1b_tv1 or smyd1a mRNA. At 72 hpf flatline mutants were identified and evaluated in regard to restored cardiac contractility. Shown are the mean percentages (+/− SD) of rescued fla embryos (n = 80) of four independent experiments. Statistical analysis was performed using one-way ANOVA (****p < .0001). (C) Measurement and statistical analysis as in (B) of ventricular FS of rescued fla mutants by injection of smyd1a- or smyd1b-mRNA at 72 hpf. Data are mean values ± SD. (D) Smyd1a specific ISH in fla mutants compared to control siblings (sib). (E) qRT-PCR analysis of smyd1a expression in control versus fla embryos.

Expression Data

Gene:	smyd1a
Fish:	WT smyd1b^zf340/zf340
Anatomical Term:	skeletal muscle
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.