FIGURE

Fig. 3

ID

ZDB-FIG-180522-5

Publication

Kimelman et al., 2017 - Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1

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Fig. 3

Regulation of ecrg4b expression by Yap1 and Wwtr1.

(A,B) ecrg4b is expressed in the presumptive epidermis of sibling embryos (A) but is not expressed in yap1;wwtr1 double mutant embryos (B). (C–F) Transgenic embryos obtained from an outcross of hemizygous HS:DN-yap or HS:CA-yap adults were heat shocked at the 4-somite stage and then raised to 18 somites. The genotype of the embryos was determined by PCR after the in situ hybridization to be either transgenic or non-transgenic control. Expression of DN-yap decreased ecrg4b expression relative to non-transgenic controls (C,D, n = 6 for each) whereas CA-yap enhanced ecrg4b expression (E,F, n = 4 for each). (G,H) Embryos analyzed using FISH probe to ecrg4b demonstrates that CA-yap increases expression of ecrg4b only in the epidermis (n = 4 for each). All embryos are at the 18-somite stage.

Expression Data

Gene:	ecrg4b
Fish:	WT zf621Tg zf622Tg yap1^bns19/bns19; wwtr1^bns35/bns35
Condition:	heat shock
Anatomical Term:	ectoderm
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf621Tg zf622Tg yap1^bns19/bns19; wwtr1^bns35/bns35
Condition:	heat shock
Observed In:	ectoderm
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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