FIGURE

Fig. 1

ID

ZDB-FIG-180522-1

Publication

Kimelman et al., 2017 - Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1

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Fig. 1

yap1;wwtr1 double mutants exhibit severely altered posterior development.

(A) Schematic showing the two mutants used in this work. TEAD BD is the TEAD binding domain, TAD is the Transcriptional Activation Domain, and WW, SH and PDZ are protein interaction domains. The black bar indicates new sequence following the frame shift caused by the mutation. (B) Time course showing the development of the posterior body defect in yap1;wwtr1 double mutants. The same sibling and double mutant embryos are photographed at all three stages. (C) Phalloidin staining shows that the somites form in the mutants but never acquire the chevron shape found in wild-type embryos. 24-somite stage (21 hpf) embryos, anterior to the left. (D) Embryos with a yap1^-/-;wwtr1^+/- genotype show a milder posterior body defect at 72 hpf (n = 15). Pericardial edema is also variably present. (E,F) Midline confocal sections of the trunk with somite 1 on the left side of a sibling (E) and a yap1;wwtr1 double mutant embryo (F) at the 24-somite stage.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	yap1^bns19/bns19; wwtr1^bns35/bns35
Observed In:	notochord pericardium somite whole organism posterior region
Stage Range:	14-19 somites to Protruding-mouth

Phenotype Detail

Acknowledgments

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