FIGURE

Fig. s4

ID
ZDB-FIG-180323-19
Publication
Herbert et al., 2017 - Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.
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Fig. s4

Coimmunoprecipitation pulls out p135 in addition to p150Glued (related to Figs. 6 and 7). (A) Image of a cell expressing a control construct for MS2-GFP, which contains a nuclear localization sequence (NLS). In the absence of any stem loop containing constructs, such as MBP, MS2-GFP localizes to the nucleus. (B) Identity of the p135 band from an anti-GFP immunoprecipitation against MS2-GFP–associated MBP mRNA granules was confirmed by comparing it to the eluted band from an immunoprecipitation using an antibody targeted against the N terminus of p150Glued, which contains its microtubule-binding domain. (C) Ribbon diagram of DCTN1 isoform 1 (p150Glued) shows a CAP-Gly microtubule-binding domain, which is lacking in DCTN1 isoform 2 (p135). (D) Kymograph analysis shows rapid back-and-forth movement of Mbp mRNA granules.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA