ZFIN Image: Herbert et al., 2017, Fig. s4

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Figure Caption

Fig. s4

Coimmunoprecipitation pulls out p135 in addition to p150^Glued (related to Figs. 6 and 7). (A) Image of a cell expressing a control construct for MS2-GFP, which contains a nuclear localization sequence (NLS). In the absence of any stem loop containing constructs, such as MBP, MS2-GFP localizes to the nucleus. (B) Identity of the p135 band from an anti-GFP immunoprecipitation against MS2-GFP–associated MBP mRNA granules was confirmed by comparing it to the eluted band from an immunoprecipitation using an antibody targeted against the N terminus of p150^Glued, which contains its microtubule-binding domain. (C) Ribbon diagram of DCTN1 isoform 1 (p150^Glued) shows a CAP-Gly microtubule-binding domain, which is lacking in DCTN1 isoform 2 (p135). (D) Kymograph analysis shows rapid back-and-forth movement of Mbp mRNA granules.

Acknowledgments

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