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ZFIN ID: ZDB-FIG-180119-30
Eneman et al., 2017 - Pituitary adenylate cyclase-activating polypeptide (PACAP) in zebrafish models of nephrotic syndrome. PLoS One   12:e0182100 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 4

PACAP morpholino suppression and PACAP-38 rescue in nephrin depleted embryos.

(A) Representative larvae of the phenotypes observed in different groups with or without PACAP (adcyap1a and adcyap1b) morpholinos injections (100 μM each). (B) Approximately 100 injected embryos per condition were live-screened at 4 dpf and assigned to a phenotype category. Compared to the control morpholino injection alone, PACAP morpholinos produced a harmful effect when injected together with the control morpholino and a devastating effect with the nphs1 morpholino. (C) Representative larvae of the phenotypes observed in different groups with human PACAP-38 injection (5μM). (D) Approximately 100 injected embryos per condition were live-screened at 4 dpf and assigned to a phenotype category. Human PACAP-38 could rescue to a great extent the PACAP morpholino injected embryos; however, they produced no beneficial effect on the nphs1 morpholino injected embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg + MO1-nphs1 + MO3-adcyap1a + MO3-adcyap1b standard conditions Day 4 pericardium edematous, exacerbated
Day 4 whole organism dead, exacerbated
Day 4 whole organism morphology, exacerbated
la2Tg + MO3-adcyap1a + MO3-adcyap1b standard conditions Day 4 pericardium edematous, abnormal
Day 4 whole organism dead, abnormal
Day 4 whole organism morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One