ZFIN ID: ZDB-FIG-180119-32
Eneman et al., 2017 - Pituitary adenylate cyclase-activating polypeptide (PACAP) in zebrafish models of nephrotic syndrome. PLoS One   12:e0182100 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 7

Quantification of thrombocytes in adriamycin treated zebrafish.

(A) LEFT: GFP-labeled thrombocytes and thrombocyte precursors are formed in the zebrafish caudal hematopoietic tissue (CHT). Representative pictures of the CHT region at 4 dpf of embryos exposed to adriamycin 0, 10 and 30 μM are shown. No obvious difference in thrombocytes was observed between the controls and the adriamycin exposed fish. RIGHT: Pixel intensity was measured using ImageJ software. The graph represents means ± SD from measurements in three embryos per condition. No significant difference was observed between adriamycin exposed and control embryos. (B) LEFT: Western blot for GFP and β-actin (loading control) was performed using total zebrafish lysates at 4 dpf. A representative blot is shown. RIGHT: Signal intensity was measured using ImageJ software. The graph represents means ± SD from measurements in two repeated experiments. No significant difference was observed between adriamycin exposed and control embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg control Day 4 thrombocyte IFL
la2Tg chemical treatment by environment: doxorubicin Day 4 thrombocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg chemical treatment by environment: doxorubicin Day 4 thrombocyte amount, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One