ZFIN ID: ZDB-IMAGE-180119-30
Figures for Eneman et al., 2017

Figure Caption/Comments:

Fig. 4

PACAP morpholino suppression and PACAP-38 rescue in nephrin depleted embryos.

(A) Representative larvae of the phenotypes observed in different groups with or without PACAP (adcyap1a and adcyap1b) morpholinos injections (100 μM each). (B) Approximately 100 injected embryos per condition were live-screened at 4 dpf and assigned to a phenotype category. Compared to the control morpholino injection alone, PACAP morpholinos produced a harmful effect when injected together with the control morpholino and a devastating effect with the nphs1 morpholino. (C) Representative larvae of the phenotypes observed in different groups with human PACAP-38 injection (5μM). (D) Approximately 100 injected embryos per condition were live-screened at 4 dpf and assigned to a phenotype category. Human PACAP-38 could rescue to a great extent the PACAP morpholino injected embryos; however, they produced no beneficial effect on the nphs1 morpholino injected embryos.

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