ZFIN ID: ZDB-FIG-180119-28
Eneman et al., 2017 - Pituitary adenylate cyclase-activating polypeptide (PACAP) in zebrafish models of nephrotic syndrome. PLoS One   12:e0182100 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 1

Phenotype and nephrin expression in nephrin depleted zebrafish.

(A) Three categories of phenotypes were defined in nephrin morpholino injected embryos: embryos without edema (normal), embryos with visible pericardial edema (white arrow) and dysmorphic or dead embryos. Pictures were taken at 5 dpf. (B) 80 embryos per condition were live-screened at 5 dpf and assigned to a phenotype category. Increasing concentrations of the injected nephrin morpholino were associated with an increasing percentage of embryos with pericardial edema, but also with increased numbers of severely dysmorphic or dead embryos. (C) RT-PCR was performed using total RNA extracted from nephrin morpholino (100 μM) and control morpholino injected embryos at 24, 48 and 72 hpf. Reduced expression of normal nephrin (389 bp) was found in the nephrin depleted versus control embryos. Moreover, injection of nephrin morpholino induced alternative splicing resulted in 272 and 1070 bp fragments, resulting from an exon deletion and a retained intron, respectively. (D) Quantitation of nphs1 RNA expression in control versus nephrin morpholino injected larvae after 24, 48 and 72 h of injection. M, marker; Mo, nephrin morpholino injected; Co, control morpholino injected.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nphs1 la2Tg + MO1-nphs1 standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg + MO1-nphs1 standard conditions Prim-5 whole organism nphs1 expression decreased amount, abnormal
Long-pec whole organism nphs1 expression decreased amount, abnormal
Protruding-mouth whole organism nphs1 expression decreased amount, abnormal
Day 5 pericardium edematous, abnormal
Day 5 whole organism dead, abnormal
Day 5 whole organism morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One