ZFIN ID: ZDB-FIG-180119-21
Schwarzer et al., 2017 - Dlx3b/4b is required for early-born but not later-forming sensory hair cells during zebrafish inner ear development. Biology Open   6(9):1270-1278 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Long-pec

Fig. 4

Delayed formation of sensory hair cells in dlx3b/4b-deficient embryos. (A-D) In comparison with control embryos at 24 hpf and 32 hpf, myo7aa expression is initially absent and subsequently restricted to the domain of the future utricular macula in dlx3b/4b mutants. (E-J) Expression of Tg(pou4f3:GAP-GFP) in control siblings and dlx3b/4b mutants at 21 hpf, 24 hpf and 48 hpf confirms delayed formation of sensory hair cells in dlx3b/4b-deficient embryos. Arrowheads indicate positions of the nascent otoliths in wild-type embryos. Note the initial onset of GFP in the prospective domain of the utricular macula and the subsequent expression in the future domain of the saccular macula in dlx3b/4b mutants. (I′,J′) Kinocilia formation appears normally in control siblings and dlx3b/4b mutants. Lateral views are shown with anterior to the left. NT, neural tube; OV, otic vesicle. Scale bars: 40 µm in D; 25 µm in J; 10 µm in J′. (K) Time course showing the mean number of pou4f3:GAP-GFP-positive hair cells in the domain of the future utricular and saccular domain of control (ctrl) and dlx3b/4b mutant embryos. dlx3b/4b-deficient embryos exhibit a significantly reduced number of sensory hair cells at 21 hpf and 24 hpf, and display significantly fewer hair cells at 48 hpf (**P<0,01; ***P<0,001; ****P<0,0001). Data are mean±s.e.m (n≥6 for each time point).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP Df(Chr12:dlx3b, dlx4b)tud70/tud70; s356tTg standard conditions 20-25 somites Not Detected otic placode IFL
Prim-5 otic vesicle IFL
Prim-5 utricle IFL
Long-pec hair cell anterior macula IFL
Long-pec hair cell posterior macula IFL
s356tTg standard conditions 20-25 somites otic placode IFL
Prim-5 immature hair cell anterior macula IFL
Prim-5 immature hair cell posterior macula IFL
Long-pec hair cell anterior macula IFL
Long-pec hair cell posterior macula IFL
myo7aa AB standard conditions Prim-5 hair cell anterior macula ISH
Prim-5 hair cell posterior macula ISH
Prim-15 hair cell anterior macula ISH
Prim-15 hair cell posterior macula ISH
Df(Chr12:dlx3b, dlx4b)tud70/tud70 (AB) standard conditions Prim-5 Not Detected hair cell anterior macula ISH
Prim-5 Not Detected hair cell posterior macula ISH
Prim-15 macula utricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
Df(Chr12:dlx3b, dlx4b)tud70/tud70 (AB) standard conditions Prim-5 hair cell anterior macula myo7aa expression absent, abnormal
Prim-5 hair cell posterior macula myo7aa expression absent, abnormal
Df(Chr12:dlx3b, dlx4b)tud70/tud70; s356tTg standard conditions 20-25 somites otic placode GFP expression absent, abnormal
20-25 somites - Long-pec otic vesicle hair cell decreased amount, abnormal
Long-pec hair cell kinocilium morphology, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open