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ZFIN ID: ZDB-FIG-171117-4
Lee et al., 2016 - Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration. PLoS One   11:e0157768 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 6 to Days 7-13

Fig. 4

Inhibition of Fgf signaling suppresses HC regeneration.

(A) A significant reduction in the number of HCs (HCS1+) regenerated after SU5402 treatment that was dose-dependent. Ctr, time-matched fish without neomycin treatment. (B) A significant reduction in the number of HCs regenerated in hsp70l:dn-fgfr1:GFP (Hsp) zebrafish neuromasts at 32°C or 37°C. AB: wild type AB fish. Ctr, time-matched transgenic fish without neomycin treatment. (C) A significant reduction in proliferation and transdifferentiation derived HCs after 20 μM SU5402 treatment for 72 hrs. ***p<0.001; **p<0.01. For all statistical analysis, n = 15 for each group. Three (for A) and two (for B,C) independent experiments were performed with similar results. (D) Fgf targets etv4 and spry4 were down-regulated by SU5402 (20 μM) in L1 neuromasts during HC regeneration. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 AB standard conditions Day 6 neuromast IHC
AB chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Day 6 neuromast IHC
AB chemical treatment by environment: neomycin, chemical treatment: SU5402 Day 6 neuromast IHC
spry4 AB standard conditions Day 6 neuromast IHC
AB chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Day 6 neuromast IHC
AB chemical treatment by environment: neomycin, chemical treatment: SU5402 Day 6 neuromast IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Day 6 neuromast etv4 expression increased amount, abnormal
Day 6 neuromast spry4 expression increased amount, abnormal
Days 7-13 neuromast hair cell amount, normal
AB chemical treatment by environment: neomycin, chemical treatment: SU5402 Day 6 neuromast etv4 expression amount, ameliorated
Day 6 neuromast spry4 expression amount, ameliorated
Days 7-13 neuromast hair cell decreased amount, abnormal
pd1Tg heat shock, chemical treatment by environment: neomycin Days 7-13 neuromast hair cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One