FIGURE

Fig. 3

ID

ZDB-FIG-171117-3

Publication

Lee et al., 2016 - Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration

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Fig. 3

Inhibition of c-Myc blocked proliferation and down-regulated vimentin during HC regeneration.

(A) A significant reduction in the number of proliferating neuromast cells (BrdU⁺) was seen 18 or 24 hrs after c-MYC inhibitor Int-H1-S6A, F8A (Myc-pep, 100 nM) treatment. Ctr, time-matched control fish without neomycin treatment. (B) c-MYC peptide inhibitor mainly blocked proliferation-derived HCs (HC-BrdU⁺) at 72 hrs. ***p<0.001; **p<0.01. A and B, n = 15 for each group. Two independent experiments were performed with similar results. (C-H) In situ hybridization of atoh1a on 5-dpf zebrafish neuromasts 18 (C-E) or 24 hrs (F-H) after neomycin treatment, with either Myc-pep or DMSO in the media. Ctr, time-matched control fish without neomycin treatment. atoh1a up-regulation by neomycin treatment was blocked in the Myc-pep treatment group (D-E, G-H). (I-Q) Vimentin was down-regulated by c-Myc during HC regeneration. 5-dpf neuromasts were labeled with vimentin and DAPI 18 hrs after neomycin treatment, with either Myc-pep (O-Q) or DMSO (L-N) in the media. No Trt, time-matched control fish without neomycin treatment (I-K). Scale bars: 10 μm.

Expression Data

Gene:	atoh1a
Antibody:	Ab6-vim
Fish:	AB
Condition:	chemical ablation: neuromast hair cell, chemical treatment by environment: neomycin
Anatomical Term:	neuromast
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical ablation: neuromast hair cell, chemical treatment by environment: neomycin
Observed In:	neuromast
Stage:	Day 5

Phenotype Detail

Acknowledgments

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