ZFIN ID: ZDB-FIG-171108-8
Juárez-Morales et al., 2016 - Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism. Neural Development   11:5 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 8

Neurotransmitter phenotypes in evx1;evx2 double mutant embryos. Lateral views of zebrafish spinal cord at 24 h (a-n). Anterior is left, dorsal up. in situ hybridization for gene or genes indicated. slc17a6 (a-d) corresponds to a mix of slc17a6b and slc17a6a probes that label glutamatergic cells; slc32a1 (e-h, formerly called viaat) labels all inhibitory cells; slc6a5 (i-l) labels glycinergic cells and gads (m & n) corresponds to a mix of gad1a and gad2 probes that labels GABAergic cells (see methods for more details). o-r Average number of cells (y-axis) expressing these markers (indicated in each case) in spinal cord region adjacent to somites 6-10 in WT embryos and evx1 and evx2 single and double mutants (x-axis) at 24 h. Values are shown as mean +/- standard deviation (values are provided in Table 1). In each case at least 5 embryos were counted. Statistically significant differences (P < 0.05) from WT values are indicated with brackets and stars. P values for all comparisons are provided in Table 1. Scale bar: 50 μm (a-n)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gad1b WT standard conditions Prim-5 spinal cord GABAergic neuron ISH
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord GABAergic neuron ISH
gad2 WT standard conditions Prim-5 spinal cord GABAergic neuron ISH
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord GABAergic neuron ISH
slc6a5 WT standard conditions Prim-5 spinal cord glycinergic neuron ISH
evx1i232/i232 standard conditions Prim-5 spinal cord glycinergic neuron ISH
evx2sa140/sa140 standard conditions Prim-5 spinal cord glycinergic neuron ISH
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord glycinergic neuron ISH
slc17a6a WT standard conditions Prim-5 spinal cord glutamatergic neuron ISH
evx1i232/i232 standard conditions Prim-5 spinal cord glutamatergic neuron ISH
evx2sa140/sa140 standard conditions Prim-5 spinal cord glutamatergic neuron ISH
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord glutamatergic neuron ISH
slc17a6b WT standard conditions Prim-5 spinal cord glutamatergic neuron ISH
evx1i232/i232 standard conditions Prim-5 spinal cord glutamatergic neuron ISH
evx2sa140/sa140 standard conditions Prim-5 spinal cord glutamatergic neuron ISH
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord glutamatergic neuron ISH
slc32a1 WT standard conditions Prim-5 spinal cord inhibitory interneuron ISH
evx1i232/i232 standard conditions Prim-5 spinal cord inhibitory interneuron ISH
evx2sa140/sa140 standard conditions Prim-5 spinal cord inhibitory interneuron ISH
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord inhibitory interneuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
evx1i232/i232 standard conditions Prim-5 spinal cord has extra parts of type glycinergic neuron, abnormal
Prim-5 spinal cord has fewer parts of type glutamatergic neuron, abnormal
Prim-5 spinal cord has normal numbers of parts of type GABAergic neuron, normal
Prim-5 ventral spinal cord interneuron specification decreased process quality, abnormal
evx2sa140/sa140 standard conditions Prim-5 spinal cord has extra parts of type glycinergic neuron, abnormal
Prim-5 spinal cord has fewer parts of type glutamatergic neuron, abnormal
Prim-5 spinal cord has normal numbers of parts of type GABAergic neuron, normal
Prim-5 ventral spinal cord interneuron specification decreased process quality, abnormal
evx2sa140/sa140 ; evx1i232/i232 standard conditions Prim-5 spinal cord has extra parts of type glycinergic neuron, abnormal
Prim-5 spinal cord has fewer parts of type glutamatergic neuron, abnormal
Prim-5 spinal cord has normal numbers of parts of type GABAergic neuron, normal
Prim-5 ventral spinal cord interneuron specification decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.