FIGURE

Fig. 4

ID

ZDB-FIG-171108-4

Publication

Juárez-Morales et al., 2016 - Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism

Other Figures

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Fig. 4

V0v cells develop into CoSA interneurons. Immunohistochemistry for EGFP in Tg(evx1:EGFP) ^SU1 (a, b & e) or Tg(evx1:EGFP) ^SU2 (c, d, f & g) embryos.^. a, f & g lateral views with dorsal up and anterior left of spinal cord at 27 h (a) or 48 h (f & g). b-e dorsal views with anterior left of zebrafish spinal cord at 30 h (b) or 48 h (c-e). a-c & f WT, (d & g) evx1;evx2 double mutant, (e) evx1 mutant injected with evx2 morpholino. b & c show increasing number of commissural axons crossing the spinal cord as development proceeds. d & e demonstrate that V0v axons are still clearly commissural in the absence of Evx1 and Evx2. f & g show magnified views of commissural ascending V0v axons. White arrows (drawn slightly to the right of the axon so that EGFP expression is still visible) indicate ascending axon trajectories. Scale bar: 50 μm (a-e) and 15 μm (f & g)

Expression Data

Gene:	EGFP
Fish:	su1Tg su2Tg evx1^i232/i232; evx2^sa140/sa140; su2Tg evx1^i232/i232; su1Tg + MO1-evx2 + MO2-evx2
Knockdown Reagents:	MO1-evx2 MO2-evx2
Anatomical Terms:	CoSA axon CoSA neuronal cell body
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	evx1^i232/i232; evx2^sa140/sa140; su2Tg evx1^i232/i232; su1Tg + MO1-evx2 + MO2-evx2
Knockdown Reagents:	MO1-evx2 MO2-evx2
Observed In:	CoSA axon extension involved in axon guidance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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