FIGURE

Fig. 2

ID

ZDB-FIG-170927-15

Publication

Madigan et al., 2017 - A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

M. leprae Triggers Myelin Dissociation

(A) Left, diagram of a spinal cord injection in an mbp:eGFP-CAAX larva (mbp), with fluorescent myelinating membrane, at 4 days post-fertilization (dpf). Transverse (middle) and sagittal (right) views of the region show the spinal cord (black), dorsal (d), and ventral (v) tracts of myelinated axons (green surrounding white axons), neuronal cell bodies (dark gray circles), and the ventral roots of spinal nerves (VR) surrounded by muscle (blue) and notochord (light gray). Arrows indicate intact myelin sheaths surrounding axons.

(B) Confocal image corresponding to (A). Scale bar, 10 μm.

(C) Representative confocal images of 4 dpf mbp larvae, 2 days post-infection (dpi) with ∼10⁴M. leprae, or ∼200 CFUs of M. marinum; arrowheads indicate myelin protrusions, quantified in (D). Scale bar, 10 μm.

(D) Mean number of myelin protrusions per animal following injection with PBS vehicle (veh), M. marinum, or M. leprae (^∗p < 0.05; one-way ANOVA, Bonferroni’s post-test).

(E) Mean bacterial burden of larvae from (C); measured by fluorescent pixel intensity like in Figure 1F.

(F and G) Representative confocal image (F) and rendering (G) of myelin protrusions in a 6 dpf larva 4 dpi with ∼10⁴M. leprae (Movie S1). Scale bar, 10 μm.

See also Table S1 and Movie S1.

Expression Data

Gene:	EGFP
Fish:	ue2Tg (AB)
Conditions:	bacterial treatment by injection: Mycobacterium leprae bacterial treatment by injection: Mycobacterium marinum
Anatomical Terms:	myelin accumulating cell membrane myelinating Schwann cell membrane oligodendrocyte membrane ventral root myelin accumulating cell
Stage Range:	Day 4 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ue2Tg (AB)
Condition:	bacterial treatment by injection: Mycobacterium leprae
Observed In:	myelin accumulating cell cell projection myelin accumulating cell membrane neuron myelin sheath
Stage Range:	Day 4 to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 170, Madigan, C.A., Cambier, C.J., Kelly-Scumpia, K.M., Scumpia, P.O., Cheng, T.Y., Zailaa, J., Bloom, B.R., Moody, D.B., Smale, S.T., Sagasti, A., Modlin, R.L., Ramakrishnan, L., A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy, 973-985.e10, Copyright (2017) with permission from Elsevier. Full text @ Cell