FIGURE

Fig. 4

ID
ZDB-FIG-170921-99
Publication
Chernyavskaya et al., 2017 - Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling
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Fig. 4

Leukocyte depletion does not reduce the induction of immune genes in uhrf1 mutants. Macrophages (A) and neutrophils (B) are enriched in the head (magnified in inset) of Tg(mpeg:mCherry;uhrf1) (A) and Tg(lysZ:dsRed;uhrf1) (B) and WT embryos at 80 and 120?hpf. Macrophages (C) and neutrophils (D) in the head region (inset in A,B) are significantly increased in uhrf1 mutants at 80 and 120?hpf. The number of embryos and clutches analyzed is indicated for each sample. (E) 0.5?mM pu.1 morpholino (MO) effectively reduces the leukocyte population. (F) RT-qPCR analysis reveals fold change in expression of immune genes from five clutches of uhrf1 and uhrf1 mutant/pu.1 morphants at 80?hpf (expression in WT embryos is shown in Fig. S5). A line connecting two dots represents one paired sample, which are siblings from a single clutch. P-values were determined by t-test (C,D) or paired t-test (F).
Expression Data
Genes:
Fish: (all 8)
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish: (all 6)
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Phenotype Detail
Acknowledgments
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