ZFIN ID: ZDB-FIG-170921-99
Chernyavskaya et al., 2017 - Loss of DNA methylation in zebrafish embryos activates retrotransposons to trigger antiviral signaling. Development (Cambridge, England)   144(16):2925-2939 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4

Leukocyte depletion does not reduce the induction of immune genes in uhrf1 mutants. Macrophages (A) and neutrophils (B) are enriched in the head (magnified in inset) of Tg(mpeg:mCherry;uhrf1) (A) and Tg(lysZ:dsRed;uhrf1) (B) and WT embryos at 80 and 120 hpf. Macrophages (C) and neutrophils (D) in the head region (inset in A,B) are significantly increased in uhrf1 mutants at 80 and 120 hpf. The number of embryos and clutches analyzed is indicated for each sample. (E) 0.5 mM pu.1 morpholino (MO) effectively reduces the leukocyte population. (F) RT-qPCR analysis reveals fold change in expression of immune genes from five clutches of uhrf1 and uhrf1 mutant/pu.1 morphants at 80 hpf (expression in WT embryos is shown in Fig. S5). A line connecting two dots represents one paired sample, which are siblings from a single clutch. P-values were determined by t-test (C,D) or paired t-test (F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 nz50Tg standard conditions Protruding-mouth head neutrophil IFL
Day 5 head neutrophil IFL
nz50Tg + MO1-spi1b standard conditions Protruding-mouth head neutrophil IFL
nz50Tg ; uhrf1hi272Tg/hi272Tg standard conditions Protruding-mouth head neutrophil IFL
Day 5 head neutrophil IFL
nz50Tg ; uhrf1hi272Tg/hi272Tg + MO1-spi1b standard conditions Protruding-mouth head neutrophil IFL
mCherry gl23Tg standard conditions Protruding-mouth head macrophage IFL
Day 5 head macrophage IFL
gl23Tg + MO1-spi1b standard conditions Protruding-mouth head macrophage IFL
gl23Tg ; uhrf1hi272Tg/hi272Tg standard conditions Protruding-mouth head macrophage IFL
Day 5 head macrophage IFL
gl23Tg ; uhrf1hi272Tg/hi272Tg + MO1-spi1b standard conditions Protruding-mouth head macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl23Tg + MO1-spi1b standard conditions Protruding-mouth head macrophage mCherry expression decreased distribution, abnormal
gl23Tg ; uhrf1hi272Tg/hi272Tg standard conditions Protruding-mouth head macrophage increased amount, abnormal
Protruding-mouth head macrophage mCherry expression increased distribution, abnormal
Protruding-mouth macrophage increased amount, abnormal
Day 5 head macrophage increased amount, abnormal
Day 5 head macrophage mCherry expression increased distribution, abnormal
gl23Tg ; uhrf1hi272Tg/hi272Tg + MO1-spi1b standard conditions Protruding-mouth head macrophage mCherry expression decreased distribution, abnormal
nz50Tg + MO1-spi1b standard conditions Protruding-mouth head neutrophil DsRed2 expression decreased distribution, abnormal
nz50Tg ; uhrf1hi272Tg/hi272Tg standard conditions Protruding-mouth head neutrophil increased amount, abnormal
Protruding-mouth head neutrophil DsRed2 expression increased distribution, abnormal
Day 5 head neutrophil increased amount, abnormal
Day 5 head neutrophil DsRed2 expression increased distribution, abnormal
nz50Tg ; uhrf1hi272Tg/hi272Tg + MO1-spi1b standard conditions Protruding-mouth head neutrophil DsRed2 expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development