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ZIRC
ZFIN ID: ZDB-FIG-170830-6
Rasouli et al., 2017 - Regulation of cardiomyocyte behavior in zebrafish trabeculation by Neuregulin 2a signaling. Nature communications   8:15281 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 6

Contractility/blood flow is required for endocardial expression of nrg2a but not the ability of cardiomyocytes to respond to nrg2a.

(ai) Maximum intensity z-projections (25–30 z-stacks, mid-sagittal sections) of Tg(kdrl:NLS-EGFP);nrg2a+/− hearts from non-treated (ac), tnnt2a MO injected (df) and BDM treated (gi) 78 hpf larvae; scale bars, 50 μm. (jl) Graphs showing cell-based Nrg2a-mRFP intensity in outer curvature (yellow box) and inner curvature (green box) of hearts from non-treated (j), tnnt2a MO (k) and BDM treated (l) 78 hpf larvae; dots represent individual Nrg2a-mRFP expressing endocardial cells. Values represent means±s.e.m.; **P≤0.01, ***P≤0.001, NS (not significant), by Student’s t-test. (m,n) 2D confocal images (mid-sagittal sections) of 78 hpf Tg(myl7:EGFP-Hsa.HRAS) (m) or Tg(myl7:EGFP-Hsa.HRAS);Tg(myl7:nrg2a-p2a-tdTomato) hearts (n) showing that myocardial overexpression of nrg2a can induce cardiomyocyte multilayering in tnnt2a morphants; asterisks and arrows indicate single-layered and multilayered ventricular walls, respectively. (or) Maximum intensity z-projections of TgBAC(cdh2:cdh2-EGFP) hearts from non-injected (o), injected with tnnt2a MO alone (p) or injected with tnnt2a MO and myl7:nrg2a-p2a-tdTomato plasmid (q,r) 96 hpf larvae. (r) 2D confocal image (sagittal section) of heart shown in q. Magnified images of dashed boxes are shown below (or); arrowheads and lozenges indicate presence and absence of Cdh2-EGFP proteins on the basal side of cardiomyocytes, respectively; At: atrium; V, ventricle; scale bars, 50 μm. (s) Schematic diagram of modulation of Nrg2a/Erbb2 signaling by cardiac contractility/blood flow.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP nrg2amn0237Gt/+ ; ubs1Tg standard conditions Protruding-mouth atrial endocardium IFL
Protruding-mouth ventricular endocardium IFL
nrg2amn0237Gt/+ ; ubs1Tg chemical treatment: Diacetylmonoxime Protruding-mouth atrial endocardium IFL
Protruding-mouth ventricular endocardium IFL
nrg2amn0237Gt/+ ; ubs1Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial endocardium IFL
Protruding-mouth ventricular endocardium IFL
s883Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
s883Tg ; bns140Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
zf517Tg standard conditions Day 4 cardiac muscle cell basal side IFL
zf517Tg + MO1-tnnt2a standard conditions Day 4 cardiac muscle cell IFL
Day 4 Not Detected cardiac muscle cell basal side IFL
mRFP1 nrg2amn0237Gt/+ ; ubs1Tg standard conditions Protruding-mouth atrial endocardium IFL
Protruding-mouth ventricular endocardium IFL
nrg2amn0237Gt/+ ; ubs1Tg chemical treatment: Diacetylmonoxime Protruding-mouth atrial endocardium IFL
Protruding-mouth ventricular endocardium IFL
nrg2amn0237Gt/+ ; ubs1Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial endocardium IFL
Protruding-mouth ventricular endocardium IFL
Tomato s883Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
s883Tg ; bns140Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nrg2amn0237Gt/+ ; ubs1Tg chemical treatment: Diacetylmonoxime Protruding-mouth atrial endocardium mRFP1 expression decreased amount, abnormal
Protruding-mouth ventricular endocardium mRFP1 expression decreased amount, abnormal
nrg2amn0237Gt/+ ; ubs1Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial endocardium mRFP1 expression decreased amount, abnormal
Protruding-mouth ventricular endocardium mRFP1 expression decreased amount, abnormal
s883Tg ; bns140Tg + MO1-tnnt2a standard conditions Protruding-mouth atrial myocardium cardiac muscle cell increased amount, abnormal
Protruding-mouth ventricular myocardium cardiac muscle cell increased amount, abnormal
zf517Tg + MO1-tnnt2a standard conditions Day 4 cardiac muscle cell EGFP expression mislocalised, abnormal
Day 4 cardiac muscle cell basal side EGFP expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.