header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-170825-7
Paksa et al., 2016 - Repulsive cues combined with physical barriers and cell-cell adhesion determine progenitor cell positioning during organogenesis. Nature communications   7:11288 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 5

Lack of the developing gut causes PGC cluster fusion.

(a) Whole-mount in situ hybridization on 30 hpf wild-type (N=420) and sox32 mutant embryos (N=89). PGCs are labelled with nanos3 (nos3, arrows) and the gut with foxa3 probe (arrowhead, missing in sox32 mutant embryos), both in blue. Unlike the separated PGC clusters in wild-type embryos, clusters are fused at the midline in sox32 mutant embryos (upper and lower panel images, respectively). Lateral (left panels) and dorsal (right panels) views are shown. (b) Cross-sections of 28 hpf sox17:dsred transgenic embryos whose gut is labelled in red and the PGCs membrane with EGFP. In control embryos (upper panel; N=5) bilateral PGC clusters form on either side of the gut tube. In embryos lacking the gut (Sox32-deficient; N=4) the PGC clusters fuse (lower panel). Nuclei counterstained with Hoechst. Scale bars, 25 μm. (c) Generation of mosaic embryos lacking Sox32 function in all cells whose endoderm is restored by providing Sox17 function to a group of cells (Scheme). The Sox32-deficient PGC clusters (green) are separated by the gut tissue at 28 hpf (red in lower panel, arrow; N=13), while in control embryos lacking endodermal tissues fused PGC clusters are observed (upper panel; N=24). Anterior is up. (d) PGC clusters in wild-type (N=20) and sox32 mutant (N=7) embryos lacking the gut tissue at 18, 24 and 28 hpf showing the dynamics of the fusion. N is the number of embryos analysed. Scale bar, 50 μm. Anterior is up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed s903Tg; s870Tg standard conditions Prim-5 gut IFL
s903Tg; s870Tg + MO2-sox32 standard conditions Prim-5 gut IFL
foxa3 sox32ta56/ta56 standard conditions Prim-15 gut ISH
GFP s903Tg; s870Tg standard conditions Prim-5 primordial germ cell IFL
s903Tg; s870Tg + MO2-sox32 standard conditions Prim-5 primordial germ cell IFL
nanos3 sox32ta56/ta56 standard conditions Prim-15 primordial germ cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s903Tg; s870Tg + MO2-sox32 standard conditions Prim-5 primordial germ cell aggregated, abnormal
sox32ta56/ta56 standard conditions 14-19 somites primordial germ cell aggregated, abnormal
Prim-5 primordial germ cell aggregated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.