FIGURE

Fig. S10

ID

ZDB-FIG-170825-15

Publication

Paksa et al., 2016 - Repulsive cues combined with physical barriers and cell-cell adhesion determine progenitor cell positioning during organogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S10

Bilateral positioning of PGC clusters does not require the notochord or blood vessles.

One-color in situ hybridization on wild-type (a-b), clo^m39 (a-d) and flh^tk241 (e-f) embryos. PGCs are stained with nos3 (a-f, arrows on the left-panel images) and endothelial cells with kdrl probe (a-d, arrowheads in a-b). Despite the lack of endothelial cells, PGCs arrive at the target (c, arrow) and form two separate clusters at 28hpf (d; dotted lines in inset) in clo mutant embryos (number of embryos examined: N=12), similar to wild-type embryos (a-b; N=60). In embryos lacking notochord (flh^tk241), PGCs form two bilateral clusters at the gonad region at 24hpf (e-f; dotted lines in inset; N=15). Scale bars 50μm. Lateral (left panels) and dorsal (right panels) views are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.