Fig. S3
Expression of Sox18Ragged-mcherry and endogenous notch1b in developing zebrafish larvae. (A) The tg(fli1a:Gal4FF;10XUAS:Sox18Ragged-mcherry) line used to perform Sox18Ragged ChIP-Seq experiments. mCherry-Sox- 18Ragged fusion protein (red) could be observed in trunk blood vasculature (green). (B) notch1b mRNA is detected in arterial endothelial cells through-out embryonic development in a pattern similar to the notch1b-15:GFP transgene. nt = neural tube, nc = notochord, DA = dorsal aorta, PCV= posterior cardinal vein. |
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Stage Range: | 20-25 somites to Prim-5 |