FIGURE

Fig. 9

ID

ZDB-FIG-170824-20

Publication

Chiang et al., 2017 - SoxF factors induce Notch1 expression via direct transcriptional regulation during early arterial development

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Fig. 9

Mouse and zebrafish arterial Notch1 expression is dependent on SOX7/18 activity. (A) Transverse sections of whole-mount in situ hybridisation for Notch1 transcript on E8.5 mouse embryos shows a reduction of Notch1 expression (asterisks) in the bulbus cordis (bc) region of the primitive heart and vitelline artery (va) of Sox7/Sox18 double knockouts. (B) At 24 hpf notch1b expression is significantly downregulated in the dorsal aorta (asterisks) of sox7/sox18 double-morphant zebrafish, whereas its signal is unaffected in the neural tube. flt1 expression is comparable between controls and sox7/sox18 double morphants, indicating that the dorsal aorta is correctly formed. (C) notch1b was barely detectable in the dorsal aorta and ISVs of sox7/18 double-knockout zebrafish (asterisks), as compared with the WT, sox7 or sox18 heterozygotes (arrows). The number of embryos showing the illustrated phenotype among the total examined is indicated. DA, dorsal aorta; PCV, posterior cardinal vein.

Expression Data

Genes:	flt1 notch1b
Fish:	WT WT + MO1-sox18 + MO1-sox7 sox7^{hu5626/hu5626}; sox18^{hu10320/hu10320}
Knockdown Reagents:	MO1-sox7 MO1-sox18
Anatomical Terms:	dorsal aorta endothelial cell intersegmental vessel neural tube
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-sox18 + MO1-sox7 sox7^{hu5626/hu5626}; sox18^{hu10320/hu10320}
Knockdown Reagents:	MO1-sox7 MO1-sox18
Observed In:	dorsal aorta intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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