ZFIN ID: ZDB-FIG-170807-16
Xu et al., 2017 - Neurons secrete miR-132-containing exosomes to regulate brain vascular integrity. Cell Research   27(7):882-897 Full text @ Cell Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 2

Involvement of Cdh5 in miR-132 knockdown-induced defects of brain vascular integrity. (A-C) Effects of miR-132 knockdown on the expression of junction proteins. Representative blots (A) and summary (B) of Western blot analyses. The experiments were repeated 3-8 times. (C) Cdh5 immunostaining in 3-dpf Tg(Flk1:eGFP) larvae. (D and E) Effects of Cdh5 knockdown on intracranial hemorrhage (D) and DAPI leakage (E) in 3-dpf larvae. The experiments were repeated 6 times and at each time > 49 embryos were examined for each group in D. 23 control embryos and 24 morphants were analyzed in E. (F) Effect of miR-132 knockdown on vsg1 expression. The experiments were repeated three times. (G, H) Representative projected confocal images (G) and summary (H) showing no significant effect of miR-132 knockdown on the pericyte coverage of brain blood vessels. 3-dpf larvae generated by crossing Ki(pdgfrβ:Gal4) with Tg(UAS:GFP;Fli1:DsRed) were used, and GFP and DsRed are expressed in pericytes and ECs, respectively. 6 control embryos and 6 miR-132 morphants were analyzed. Scale bar, 25 μm (C), 50 μm (G). Error bars, SEM. n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired two-tailed Student's t-test for B, D, E, F and H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 Ab1-cdh5 AB standard conditions Protruding-mouth whole organism WB
Ab1-cdh5 AB + MO1-mir132-1 standard conditions Protruding-mouth whole organism WB
Ab1-cdh5 s843Tg standard conditions Protruding-mouth brain vasculature IHC
Ab1-cdh5 s843Tg + MO1-mir132-1 standard conditions Protruding-mouth brain vasculature IHC
DsRedx ion33dTg ; c369Tg ; um13Tg standard conditions Protruding-mouth endothelial cell IFL
ion33dTg ; c369Tg ; um13Tg + MO1-mir132-1 standard conditions Protruding-mouth endothelial cell IFL
GAL4 ion33dTg ; c369Tg ; um13Tg standard conditions Protruding-mouth pericyte IHC
ion33dTg ; c369Tg ; um13Tg + MO1-mir132-1 standard conditions Protruding-mouth pericyte IHC
plvapb AB standard conditions Protruding-mouth whole organism RTPCR
AB + MO1-mir132-1 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mir132-1 standard conditions Protruding-mouth whole organism cdh5 expression decreased amount, abnormal
Protruding-mouth whole organism plvapb expression decreased amount, abnormal
ion33dTg ; c369Tg ; um13Tg + MO1-mir132-1 standard conditions Protruding-mouth transcytosis process quality, normal
s843Tg + MO1-mir132-1 standard conditions Protruding-mouth brain vasculature cdh5 expression decreased amount, abnormal
s843Tg + MO2-cdh5 standard conditions Protruding-mouth cranial vasculature hemorrhagic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Res.