FIGURE

Fig. 1

ID

ZDB-FIG-170615-10

Publication

Liu et al., 2017 - Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action

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Fig. 1

The igfbp5a gene is specifically expressed in NaR cells. (a) Wild type zebrafish embryos were raised in embryo rearing solution containing the indicated [Ca²⁺] from 0 to 120?hpf (hours post fertilization) and analyzed by whole-mount in situ hybridization using the indicated probes. Images shown are the yolk sac region. Scale bar?=?50??m. Unless specified otherwise, all in situ hybridization images shown hereafter are lateral views, anterior to the left and dorsal up. (b?e) The trpv5/6 and igfbp5a genes respond differentially to [Ca²⁺] changes. The experimental design is shown in (b). The mRNA levels of igfbp5a (c) and trpv5/6 (d) were measured by qPCR and normalized by the ?-actin mRNA levels. Data shown are mean?�?SEM, n?=?3. Different letters indicate significant differences at p?<?0.05. (e) The larvae described in (b) were analyzed by in situ hybridization using the indicated probes. Representative images are shown.

Expression Data

Genes:	atp6v1aa igfbp5a trpv6
Fish:	WT
Conditions:	high calcium low calcium
Anatomical Terms:	NaK ionocyte vH ionocyte
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	low calcium
Observed In:	NaK ionocyte
Stage:	Day 5

Phenotype Detail

Acknowledgments

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