FIGURE

Fig. 1

ID

ZDB-FIG-170615-10

Publication

Liu et al., 2017 - Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

The igfbp5a gene is specifically expressed in NaR cells. (a) Wild type zebrafish embryos were raised in embryo rearing solution containing the indicated [Ca²⁺] from 0 to 120 hpf (hours post fertilization) and analyzed by whole-mount in situ hybridization using the indicated probes. Images shown are the yolk sac region. Scale bar = 50 μm. Unless specified otherwise, all in situ hybridization images shown hereafter are lateral views, anterior to the left and dorsal up. (b–e) The trpv5/6 and igfbp5a genes respond differentially to [Ca²⁺] changes. The experimental design is shown in (b). The mRNA levels of igfbp5a (c) and trpv5/6 (d) were measured by qPCR and normalized by the β-actin mRNA levels. Data shown are mean ± SEM, n = 3. Different letters indicate significant differences at p < 0.05. (e) The larvae described in (b) were analyzed by in situ hybridization using the indicated probes. Representative images are shown.

Expression Data

Genes:	atp6v1aa igfbp5a trpv6
Fish:	WT
Conditions:	high calcium low calcium
Anatomical Terms:	NaK ionocyte vH ionocyte
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	low calcium
Observed In:	NaK ionocyte
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.