FIGURE

Fig. 5

ID

ZDB-FIG-170615-13

Publication

Liu et al., 2017 - Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action

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Fig. 5

Dynamics of low [Ca²⁺]-induced NaR cell proliferation. Tg(igfbp5a:GFP) larvae (72?hpf) were transferred to the embryo rearing solution containing 0.001?mM [Ca²⁺]. Images were recorded from the same individual hourly and NaR cells numbers were determined. (a) Representative images at the indicated time points. The box shows two GFP-expressing NaR cells going through two divisions. The arrow indicates a NaR cell disappearing between 102?108?hpf. The arrowhead indicates the appearance of a new GFP-expressing cell around 86?90?hpf. (b) Average NaR cell number at the indicated time points. Values are mean?�?SEM, n?=?2. (c) NaR cell proliferation index at the indicated time periods. NaR cell proliferation index (%)?=?[(N_t???N_t?1)/N_t?1] * 100. N_t is the NaR cell number at the time of interest. N_t?1 is the NaR cell number an hour earlier. (d) Cell cycle analysis results. Left panel is a representative cell cycle histogram. Quantitative results are shown in the right panel. Values shown are mean?�?SEM, n?=?3, *p?<?0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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