header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-170516-38
Goto et al., 2017 - FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT. Development (Cambridge, England)   144(8):1412-1424 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 75%-epiboly to Prim-5

Fig. 1

FGF is required for post-gastrula mesoderm induction and repression of the NMP state. (A-E) Zebrafish embryos from an HS:dnfgfr1 hemizygous outcross were heat shocked at the 12- or 18-somite stage and analyzed for myoD expression at 24 hpf. Wild-type siblings exhibit ∼31 somites (A,C,E), whereas HS:dnfgfr1 embryos heat shocked at 12 somites have 16 somites and those heat shocked at 18 somites have 22 somites (B,D,E). The number of embryos showing the illustrated phenotype among the total number examined is indicated. Error bars indicate s.d. *P<0.001 using unpaired t-test. (F-I') Expression of ntla (arrowheads) indicates that there is no posterior loss of notochord after FGF inhibition at the 12- or 18-somite stage. (J,K) Embryos heat shocked at 50% epiboly (just at the start of gastrulation) and fixed 3 h after heat shock show a near complete loss of ntla expression. (L,M) Embryos heat shocked at the 8-somite stage and fixed 3 h later exhibit an expansion of ntla into lateral and anterior domains (arrowhead). (N,O) Inhibition of FGF signaling at the 8-somite stage also leads to an expansion of sox2 3 h after the heat shock. (A-D,F,G,H,I) Lateral view, anterior left; (F′,G′,H′,I′,L-O) dorsal view, anterior left; (J,K) vegetal view, dorsal top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 pd1Tg/+ control Prim-5 somite ISH
pd1Tg/+ heat shock Prim-5 somite ISH
sox2 pd1Tg/+ control 1-4 somites notochord ISH
pd1Tg/+ heat shock 1-4 somites notochord ISH
tbxta pd1Tg/+ control 75%-epiboly margin ISH
1-4 somites notochord ISH
5-9 somites notochord ISH
14-19 somites notochord ISH
pd1Tg/+ heat shock 75%-epiboly margin ISH
1-4 somites notochord ISH
5-9 somites notochord ISH
14-19 somites notochord ISH
vimr1 tbx16b104/b104 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
vimr2 tbx16b104/b104 standard conditions 14-19 somites notochord ISH
14-19 somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pd1Tg/+ heat shock 75%-epiboly margin tbxta expression decreased amount, abnormal
1-4 somites notochord tbxta expression spatial pattern, abnormal
1-4 somites notochord sox2 expression spatial pattern, abnormal
Prim-5 somite decreased amount, abnormal
tbx16b104/b104 standard conditions 14-19 somites tail bud vimr1 expression increased distribution, abnormal
14-19 somites tail bud vimr2 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development