ZFIN ID: ZDB-FIG-170420-8
Zhu et al., 2017 - Vegfa Impacts Early Myocardium Development in Zebrafish. International Journal of Molecular Sciences   18(2) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 1

Vascular endothelial growth factor Aa (vegfaa) deficiency disrupts myocardial and endocardial morphogenesis. (A–I) Fluorescence micrographs of the hearts of wild-type (A–C), vegfaa mutant (D–F), and vegfaa morphants (G–I) at 96 hpf (hours post-fertilization) with the background of Tg(myl7:mCherry)/Tg(kdrl:EGFP) transgenic fish. The whole heart is substantially small and linearized in the mutant and the morphant, and no cavity is visible inside the heart lumen. Scale bar: 50 μm (A– I); (J,K) In situ hybridization of vegfaa in wild-type embryos at 18-somite stage. vegfaa is expressed in anterior lateral plate mesoderm as indicated by the arrow. (J) Lateral views, (K) Dorsal views with the anterior at the top. Scale bar: 200 μm (J,K). WT, wild-type; mo, morpholino

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP la116Tg ; sd7Tg control Day 4 endocardium IHC
la116Tg ; sd7Tg + MO9-vegfaa control Day 4 endocardium IHC
la116Tg ; sd7Tg ; vegfaazf1090/zf1090 control Day 4 endocardium IHC
mCherry la116Tg ; sd7Tg control Day 4 myocardium IHC
la116Tg ; sd7Tg + MO9-vegfaa control Day 4 myocardium IHC
la116Tg ; sd7Tg ; vegfaazf1090/zf1090 control Day 4 myocardium IHC
vegfaa AB control 14-19 somites anterior lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la116Tg ; sd7Tg + MO9-vegfaa control Day 4 atrium hypotrophic, abnormal
Day 4 cardiac ventricle hypotrophic, abnormal
Day 4 endocardium endothelial cell accumulation heart anatomical space, abnormal
Day 4 heart linear, abnormal
Day 4 heart structure, abnormal
Day 4 heart looping process quality, abnormal
Day 4 heart tube formation decreased occurrence, abnormal
Day 4 heart valve malformed, abnormal
la116Tg ; sd7Tg ; vegfaazf1090/zf1090 control Day 4 atrium hypotrophic, abnormal
Day 4 cardiac ventricle hypotrophic, abnormal
Day 4 endocardium endothelial cell accumulation heart anatomical space, abnormal
Day 4 heart linear, abnormal
Day 4 heart structure, abnormal
Day 4 heart looping process quality, abnormal
Day 4 heart tube formation decreased occurrence, abnormal
Day 4 heart valve malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.