FIGURE

Fig. 6

ID

ZDB-FIG-170413-19

Publication

Mandal et al., 2017 - Wnt signaling balances specification of the cardiac and pharyngeal muscle fields

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Manipulation of Wnt signaling affects the overlap of nkx2.5+ and tcf21+ domains within the ALPM. (A–H) Tg(nkx2.5:ZsYellow) control sibling embryos and embryos with increased and decreased Wnt signaling at the SP stage. (A, B, E, F) Two-color ISH of zsyellow (nkx2.5; brownish red) and tcf21 (blue) at the 14 s and 18 s stages shows expansion of the lateral nkx2.5+ domains into the anterior tcf21+ domain after increased Wnt signaling. Numbers in E indicate the three tcf21+ fields depicted in Fig. 5. (C, D, G, H) Decreasing Wnt signaling prior to gastrulation causes a decrease in the overlap of nkx2.5 and tcf21 at the 14 s and 18 s stages. Significant overlap between nkx2.5 and tcf21 could not be detected at the 14 s stage in embryos when with Wnt signaling was inhibited prior to gastrulation. Images are dorsal views with anterior to left. Brackets indicate overlap of nkx2.5+ and tcf21+ domains. For each group > 15 embryos were examined. Scale bar indicates 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 143, Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S., Wnt signaling balances specification of the cardiac and pharyngeal muscle fields, 32-41, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.