Fig. 7
- ID
- ZDB-FIG-170413-20
- Publication
- Mandal et al., 2017 - Wnt signaling balances specification of the cardiac and pharyngeal muscle fields
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Manipulation of endogenous Wnt signaling affects the size of the nkx2.5+ and tcf21+ domains. (A, B, D, E) Tg(nkx2.5:ZsYellow);Tg(tcf21:nucEGFP) control, Tcf7l1 depleted, and Wnt8a depleted embryos at the 18 s stage. Tcf7l1 depletion expands the total anterior tcf21+ progenitor domain length (green brackets) as well as the overlapping expression (yellow brackets) within the ALPM. (C) Graph depicting measurements of the total tcf21+ progenitors and overlap with nkx2.5+ progenitors from Tcf7l1 depleted embryos. Control embryos n = 10, Tcf7l1 depleted embryos n = 9. (D, E) Wnt8a depletion reduces the overall length of the tcf21+ domain and the overlap with the nkx2.5+ progenitor domain. (F) Graph depicting measurements of the total tcf21+ progenitors and overlap with nkx2.5+ progenitors from Wnt8a depleted embryos. Control embryos n = 8, Wnt8a depleted embryos n = 10. Green brackets - anterior tcf21+ progenitors. Red brackets - nkx2.5+ progenitors. Yellow brackets – regions of overlap between tcf21+ and nkx2.5+ domains. Scale bar indicates 100 μm. |
Reprinted from Mechanisms of Development, 143, Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S., Wnt signaling balances specification of the cardiac and pharyngeal muscle fields, 32-41, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.