ZFIN Image: Mandal et al., 2017, Fig. 6

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Figure Caption

Fig. 6

Manipulation of Wnt signaling affects the overlap of nkx2.5+ and tcf21+ domains within the ALPM. (A–H) Tg(nkx2.5:ZsYellow) control sibling embryos and embryos with increased and decreased Wnt signaling at the SP stage. (A, B, E, F) Two-color ISH of zsyellow (nkx2.5; brownish red) and tcf21 (blue) at the 14 s and 18 s stages shows expansion of the lateral nkx2.5+ domains into the anterior tcf21+ domain after increased Wnt signaling. Numbers in E indicate the three tcf21+ fields depicted in Fig. 5. (C, D, G, H) Decreasing Wnt signaling prior to gastrulation causes a decrease in the overlap of nkx2.5 and tcf21 at the 14 s and 18 s stages. Significant overlap between nkx2.5 and tcf21 could not be detected at the 14 s stage in embryos when with Wnt signaling was inhibited prior to gastrulation. Images are dorsal views with anterior to left. Brackets indicate overlap of nkx2.5+ and tcf21+ domains. For each group > 15 embryos were examined. Scale bar indicates 100 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 143, Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S., Wnt signaling balances specification of the cardiac and pharyngeal muscle fields, 32-41, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.