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ZFIN ID: ZDB-FIG-170321-23
Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics   13:e1006564 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 8-cell to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 8-cell to Day 5

Fig. 1

Zebrafish stat3 mutants generated using TALEN method are strong/null alleles.

(A) Design of TALEN pair targeting zebrafish stat3 gene (yellow filled text). (B) Sequence alignments and schematics of Stat3 proteins encoded by stl27, stl28 and sa15744 alleles. (C) Expression patterns of stat3 transcripts detected by WISH in WT and MZstat3 embryos (lateral views). Arrowhead, nm, neuromast. (D) RT-PCR analysis of stat3 RNA levels in WT embryos at 1.3, 6, 8.3 and 24 hpf. gapdh was used as an internal control. (E) qRT-PCR analysis of stat3 transcript levels in WT and MZstat3 embryos normalized to gapdh. (F) Western blot detecting total Stat3 in WT, MZstat3 and MZstat3 embryos overexpressing Flag-tagged Stat3 at 6 hpf. β-actin was used as a loading control. See also S1 Fig.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
stat3 WT standard conditions 8-cell blastomere ISH
8-cell whole organism RTPCR
8-cell whole organism ISH
Ab7-stat3 Shield whole organism WB
Shield whole organism RTPCR
75%-epiboly whole organism ISH
75%-epiboly whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-15 head ISH
Day 5 head ISH
Day 5 whole organism ISH
stat3stl27/stl27 standard conditions 8-cell blastomere ISH
8-cell whole organism RTPCR
8-cell whole organism ISH
Shield whole organism RTPCR
Ab7-stat3 Shield whole organism WB
75%-epiboly whole organism ISH
75%-epiboly whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-15 head ISH
Day 5 head ISH
Day 5 neuromast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
stat3stl27/stl27 standard conditions 8-cell blastomere stat3 expression decreased amount, abnormal
8-cell whole organism stat3 expression decreased amount, abnormal
8-cell whole organism stat3 expression decreased amount, abnormal
Shield whole organism stat3 expression decreased amount, abnormal
Shield whole organism stat3 expression decreased amount, abnormal
75%-epiboly whole organism stat3 expression decreased amount, abnormal
75%-epiboly whole organism stat3 expression decreased amount, abnormal
Prim-5 whole organism stat3 expression decreased amount, abnormal
Prim-15 head stat3 expression decreased amount, abnormal
Day 5 head stat3 expression decreased amount, abnormal
Day 5 neuromast stat3 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.