FIGURE

Fig. 6

ID

ZDB-FIG-170321-28

Publication

Liu et al., 2017 - Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation

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Fig. 6

Stat3 promotes post-MBT cell divisions but does not regulate cell division orientation during zebrafish embryogenesis.

(A) Experimental design for post-MBT cell cycle analyses. Embryos labeled ubiquitously with histone2B-RFP (H2B-RFP) were mosaically injected with memCherry or mGFP + stat3-F mRNA at 8-cell stage for lineage tracing. Labeled clones (B and C) within the same embryo were monitored using confocal time-lapse microscopy (See also Materials and methods). (D) Analyses of cell cycle lengths for Cycle 11–13 in WT, WT overexpressing Stat3-F, MZstat3, and MZstat3 overexpressing Stat3-F embryos. (E-G) Cell division orientation in dorsal neuroectoderm in WT (E) and MZstat3 (F) embryos during 1–3 somite stages, with 0 degrees representing mediolaterally aligned cell divisions and 90 degrees representing anteroposteriorly aligned cell divisions, quantified in G. *p<0.05, **p<0.01, ****p<0.0001, n.s. = non-significant, error bars = SEM. See also S7 Fig.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stat3^stl27/stl27
Observed In:	mitotic cell cycle
Stage Range:	High to Sphere

Phenotype Detail

Acknowledgments

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