FIGURE

Fig. 5

ID

ZDB-FIG-170315-11

Publication

Zhang et al., 2015 - G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition

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Fig. 5

7α-25-OHC stimulation facilitates HSPC development in zebrafish. (A) Expression of ve-cadherin increases in clotrimazole-treated embryos. (B) Transition of HE to HSPCs or non-hemogenic ECs after DMSO or clotrimazole treatment beginning at 30 hpf. White asterisk indicates the same cell in different frames. (C) Quantification of HE (flk1⁺cmyb⁺, yellow cells in B) differentiating to HSPCs (flk1⁻cmyb⁺, green cells in B) or non-hemogenic ECs (flk1⁺cmyb⁻, red cells in B) (n = 5). Error bar represents SD. (D, E) runx1 expression in embryos treated with 7α-25-OHC or clotrimazole and examined at 36 hpf by WISH (D) and western blotting (E). Red arrowheads in A and D label the staining signals in AGM.

Expression Data

Genes:	cdh5 EGFP mCherry myb runx1
Fish:	TU s896Tg; zf169Tg
Conditions:	chemical treatment by injection: 7alpha,25-dihydroxycholesterol chemical treatment: clotrimazole
Anatomical Terms:	hematopoietic multipotent progenitor cell ventral wall of dorsal aorta whole organism
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU s896Tg; zf169Tg
Conditions:	chemical treatment by injection: 7alpha,25-dihydroxycholesterol chemical treatment: clotrimazole
Observed In:	endothelial to hematopoietic transition ventral wall of dorsal aorta whole organism
Stage Range:	Prim-15 to Prim-25

Phenotype Detail

Acknowledgments

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