FIGURE

Fig. S3

ID

ZDB-FIG-170315-15

Publication

Zhang et al., 2015 - G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition

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Fig. S3

gpr183 is expressed in the hemogenic endothelium (HE) after 24 hpf, and is essential for adult hematopoiesis in zebrafish.(A) Time-course expression of gpr183 ranging from one-cell stage to 36 hpf shows that gpr183 is not expressed before 24 hpf. (B) Frozen sections of the embryos after WISH show that gpr183 is expressed in the AGM at about 26 hpf. (C) gpr183 expression in sorted ECs, HEs and HSPCs at 28 hpf by qPCR. Error bar, ±s. d. *p<0.05, ***p<0.001. (D) gpr183 expression using double fluorescence in situ hybridization (dFISH) in 30hpf Tg(fli1a:eGFP) embryo. (E) gpr183 is not expressed in gpr183^-/- embryos.

Expression Data

Genes:	EGFP gpr183a
Fish:	AB/TU TU gpr183a^ioz9/ioz9 (AB/TU) y1Tg ioz1Tg; s896Tg
Anatomical Terms:	angioblastic mesenchymal cell hematopoietic multipotent progenitor cell ventral wall of dorsal aorta
Stage Range:	1-cell to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gpr183a^ioz9/ioz9 (AB/TU)
Observed In:	ventral wall of dorsal aorta
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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