FIGURE

Fig. 1

ID

ZDB-FIG-170315-7

Publication

Zhang et al., 2015 - G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition

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Fig. 1

RNA-deep sequencing analysis of gene expression in ECs, HE and HSPCs from Tg(flk1:mCherry/runx1:en-GFP) transgenic embryos. (A) Fluorescence signals in AGM and thymus of the indicated transgeneic lines showing that Tg(runx1:en-GFP) is able to label HE and HSPCs. White arrowheads denote HE or emerging HSPCs in AGM. (B) Flowchart of sorting and RNA-Seq analysis of the indicated cells from 28 hpf Tg(flk1:mCherry/runx1:en-GFP) embryos. (C) RNA-Seq analysis results showing the numbers of differentially expressed genes in sorted ECs, HECs and HSPCs from 28 hpf Tg(flk1:mCherry/runx1:en-GFP) embryos. (D) Heat-map analysis of selected genes based on RNA-Seq data.

Expression Data

Genes:	DsRed GFP mCherry
Fish:	hkz07tTg; ioz1Tg ioz1Tg; s896Tg ioz1Tg; zf411Tg
Anatomical Terms:	angioblastic mesenchymal cell endothelial cell hematopoietic multipotent progenitor cell thymus ventral wall of dorsal aorta
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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