ZFIN ID: ZDB-FIG-170302-13
Chen et al., 2017 - Cilia Control Vascular Mural Cell Recruitment in Vertebrates. Cell Reports   18:1033-1047 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3

Notch Activation Is Required for Arterial vMC Recruitment

(A) Pharmacological and genetic inhibition of Notch signaling reduces vMC recruitment around the DA. Confocal images of partial z-projections of the trunk region (somite 8–14) of Tg(kdrl:egfp)s843Tg(acta2:mCherry)uto5 embryos at 80 hpf (merged and single channels) treated with DAPT or co-injected with notch1b and notch3 morpholinos. Scatter plots show the number of vMCs counted along 500 μm of the DA at 80 hpf. n = 27, 29, and 19 embryos. Data are represented as mean ± SD. Stars represent the results of one-way ANOVA-Dunnett’s post hoc test (p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). g, gut.

(B) Hey2/grl is required for vMC recruitment. Confocal images of partial z-projections of the trunk region (somite 8–14) of Tg(kdrl:egfp)s843;Tg(acta2:mCherry)uto5 embryos at 80 hpf (merged and single channels) after grl knockdown by morpholinos injection. Compared to controls, grl morphants lack vMC coverage around the DA (arrows). Scatter plots show the quantification of vMC number per 500 μm of the DA at 3 dpf. n = 23 and 17 embryos. Data are represented as mean ± SD. Stars represent the results of unpaired t tests of mean difference = 0 (p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP s843Tg ; uto5Tg control Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth posterior cardinal vein IFL
s843Tg ; uto5Tg chemical treatment: DAPT Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth posterior cardinal vein IFL
s843Tg ; uto5Tg + MO1-hey2 control Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth posterior cardinal vein IFL
s843Tg ; uto5Tg + MO1-notch3 + MO2-notch1b control Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth posterior cardinal vein IFL
mCherry s843Tg ; uto5Tg control Protruding-mouth dorsal aorta anatomical region IFL
Protruding-mouth dorsal aorta vascular associated smooth muscle cell IFL
Protruding-mouth gut IFL
s843Tg ; uto5Tg chemical treatment: DAPT Protruding-mouth Not Detected dorsal aorta anatomical region IFL
Protruding-mouth Not Detected dorsal aorta vascular associated smooth muscle cell IFL
Protruding-mouth gut IFL
s843Tg ; uto5Tg + MO1-hey2 control Protruding-mouth Not Detected dorsal aorta anatomical region IFL
Protruding-mouth Not Detected dorsal aorta vascular associated smooth muscle cell IFL
Protruding-mouth gut IFL
s843Tg ; uto5Tg + MO1-notch3 + MO2-notch1b control Protruding-mouth Not Detected dorsal aorta anatomical region IFL
Protruding-mouth Not Detected dorsal aorta vascular associated smooth muscle cell IFL
Protruding-mouth gut IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s843Tg ; uto5Tg chemical treatment: DAPT Protruding-mouth dorsal aorta anatomical region mCherry expression absent, abnormal
Protruding-mouth dorsal aorta vascular associated smooth muscle cell decreased amount, abnormal
Protruding-mouth dorsal aorta vascular associated smooth muscle cell mCherry expression absent, abnormal
s843Tg ; uto5Tg + MO1-hey2 control Protruding-mouth dorsal aorta anatomical region mCherry expression absent, abnormal
Protruding-mouth dorsal aorta vascular associated smooth muscle cell decreased amount, abnormal
Protruding-mouth dorsal aorta vascular associated smooth muscle cell mCherry expression absent, abnormal
s843Tg ; uto5Tg + MO1-notch3 + MO2-notch1b control Protruding-mouth dorsal aorta anatomical region mCherry expression absent, abnormal
Protruding-mouth dorsal aorta vascular associated smooth muscle cell decreased amount, abnormal
Protruding-mouth dorsal aorta vascular associated smooth muscle cell mCherry expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.