ndrg4 morphant and mutant phenotype.
(A-C) Overall morphology of a control embryo (A), ndrg4 morphant embryo (B), ndrg4 MO+ndrg4 mRNA co-injected embryo (C), at 48hpf. (B) ndrg4 morphant embryo displays smaller head and eyes and is slightly thinner. (C) Co-injection of ndrg4 MO and mRNA rescue this phenotype. Scale bars = 500μm. (D-F) Acetylated tubulin and sodium channels staining of a ndrg4 MO+mRNA co-injected embryo showing clustered sodium channels along the PLLn (34.2 ±3.2; n = 12 embryos) similar to controls. Scale bar = 5μm. (G) Sodium channels staining in sox10::mRFP transgenic line in controls and ndrg4 morphants. Arrowheads indicate the clustering of sodium channels while arrows point to nodal gaps. Note the absence of sodium channels clustering at the nodes in ndrg4 morphants. Scale bar = 5 μm. (H-J) Snap25 immunostaining in HuC::GFP larvae at 3dpf. (H) Control embryos show Snap25 expression in PLLg neurons (arrows). (I) A reduced Snap25 expression was observed within the PLLg in ndrg4 morphants. (J) Rescue of Snap25 expression in the PLLg in ndrg4MO+mRNA co-injected embryos. Scale bars = 5μm. (K) Snap25 expression in the PLLg of control and ndrg4-/- embryos. Note the significant decrease in the expression of Snap25 within the PLLg of ndrg4-/- in comparison to controls.