ZFIN ID: ZDB-FIG-170222-28
Fontenas et al., 2016 - Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish. PLoS Genetics   12:e1006459 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 4

Fig. S1

ndrg4 morphant and mutant phenotype.

(A-C) Overall morphology of a control embryo (A), ndrg4 morphant embryo (B), ndrg4 MO+ndrg4 mRNA co-injected embryo (C), at 48hpf. (B) ndrg4 morphant embryo displays smaller head and eyes and is slightly thinner. (C) Co-injection of ndrg4 MO and mRNA rescue this phenotype. Scale bars = 500μm. (D-F) Acetylated tubulin and sodium channels staining of a ndrg4 MO+mRNA co-injected embryo showing clustered sodium channels along the PLLn (34.2 ±3.2; n = 12 embryos) similar to controls. Scale bar = 5μm. (G) Sodium channels staining in sox10::mRFP transgenic line in controls and ndrg4 morphants. Arrowheads indicate the clustering of sodium channels while arrows point to nodal gaps. Note the absence of sodium channels clustering at the nodes in ndrg4 morphants. Scale bar = 5 μm. (H-J) Snap25 immunostaining in HuC::GFP larvae at 3dpf. (H) Control embryos show Snap25 expression in PLLg neurons (arrows). (I) A reduced Snap25 expression was observed within the PLLg in ndrg4 morphants. (J) Rescue of Snap25 expression in the PLLg in ndrg4MO+mRNA co-injected embryos. Scale bars = 5μm. (K) Snap25 expression in the PLLg of control and ndrg4-/- embryos. Note the significant decrease in the expression of Snap25 within the PLLg of ndrg4-/- in comparison to controls.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-snap25 IHC WT control Day 4 posterior lateral line ganglion
IHC WT + CRISPR1-ndrg4 standard conditions Day 4 posterior lateral line ganglion
IHC knu3Tg control Protruding-mouth posterior lateral line ganglion neuron
IHC knu3Tg + MO2-ndrg4 control Protruding-mouth posterior lateral line ganglion neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-ndrg4 standard conditions Day 4 posterior lateral line ganglion Ab3-snap25 labeling decreased distribution, abnormal
WT + MO2-ndrg4 standard conditions Long-pec eye decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec whole organism decreased width, abnormal
knu3Tg + MO2-ndrg4 control Protruding-mouth posterior lateral line ganglion neuron Ab3-snap25 labeling decreased distribution, abnormal
vu234Tg + MO2-ndrg4 standard conditions Day 4 clustering of voltage-gated calcium channels decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.