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ZIRC
ZFIN ID: ZDB-FIG-170222-23
Fontenas et al., 2016 - Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish. PLoS Genetics   12:e1006459 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 3

ndrg4 is not required for axonal outgrowth or early Schwann cell development.

Acetylated tubulin expression in control (A), ndrg4 mutant (C) and morphant (B) embryos at 4 dpf showing the PLLn nerve. Scale bar = 45μm. (d-F) Whole mount in situ hybridization of a (d) control embryo, ndrg4 mutant (F) and ndrg4 morphant (E) showing sox10 expression in PLLn SCs (arrows) at 3 dpf. Scale bar = 200μm. Lateral view of a control foxd3::GFP embryo (G), a ndrg4 morphant (H) at 3 dpf showing SCs (arrows) along the PLLn. Transmission electron micrographs showing cross-section through (I) control and ndrg4 mutant (J). Control PLLn shows an average of 10.7 myelinated axons (blue asterisks). (J) An average of 5.36 myelinated axons (blue asterisks) is observed in the ndrg4 mutant’s PLLn. (S: Schwann cell). Scale bars = 0.5μm. (K,L) Quantification of the total number of axons and the number myelinated axons in controls, ndrg4 mutants and ndrg4 morphants. NS: Non Significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP zf15Tg control Protruding-mouth myelinating Schwann cell IFL
zf15Tg + MO2-ndrg4 standard conditions Protruding-mouth myelinating Schwann cell IFL
sox10 WT control Protruding-mouth posterior lateral line nerve immature Schwann cell ISH
WT + CRISPR1-ndrg4 standard conditions Protruding-mouth posterior lateral line nerve immature Schwann cell ISH
WT + MO2-ndrg4 standard conditions Protruding-mouth posterior lateral line nerve immature Schwann cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-ndrg4 standard conditions Day 4 myelination in peripheral nervous system disrupted, abnormal
Day 4 myelination of posterior lateral line nerve axons decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.