FIGURE

Fig. 3

ID

ZDB-FIG-170222-23

Publication

Fontenas et al., 2016 - Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish

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Fig. 3

ndrg4 is not required for axonal outgrowth or early Schwann cell development.

Acetylated tubulin expression in control (A), ndrg4 mutant (C) and morphant (B) embryos at 4 dpf showing the PLLn nerve. Scale bar = 45μm. (d-F) Whole mount in situ hybridization of a (d) control embryo, ndrg4 mutant (F) and ndrg4 morphant (E) showing sox10 expression in PLLn SCs (arrows) at 3 dpf. Scale bar = 200μm. Lateral view of a control foxd3::GFP embryo (G), a ndrg4 morphant (H) at 3 dpf showing SCs (arrows) along the PLLn. Transmission electron micrographs showing cross-section through (I) control and ndrg4 mutant (J). Control PLLn shows an average of 10.7 myelinated axons (blue asterisks). (J) An average of 5.36 myelinated axons (blue asterisks) is observed in the ndrg4 mutant’s PLLn. (S: Schwann cell). Scale bars = 0.5μm. (K,L) Quantification of the total number of axons and the number myelinated axons in controls, ndrg4 mutants and ndrg4 morphants. NS: Non Significant.

Expression Data

Genes:	GFP sox10
Fish:	WT WT + CRISPR1-ndrg4 WT + MO2-ndrg4 zf15Tg zf15Tg + MO2-ndrg4
Knockdown Reagents:	CRISPR1-ndrg4 MO2-ndrg4
Anatomical Terms:	myelinating Schwann cell posterior lateral line nerve myelinating Schwann cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-ndrg4
Knockdown Reagent:	CRISPR1-ndrg4
Observed In:	myelination in peripheral nervous system myelination of posterior lateral line nerve axons
Stage:	Day 4

Phenotype Detail

Acknowledgments

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