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ZFIN ID: ZDB-FIG-170213-3
Serbanovic-Canic et al., 2017 - Zebrafish Model for Functional Screening of Flow-Responsive Genes. Arteriosclerosis, Thrombosis, and Vascular Biology   37(1):130-143 Full text @ Arterio., Thromb., and Vas. Bio.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 5

Zebrafish functional screening of putative apoptotic regulators. A, Zebrafish embryos (flk1:EGFP-NLS embryos; green endothelial cell [EC] nuclei) were injected with morpholinos oligonucleotides (MOs) targeting candidate genes or a nontargeting control MO (indicated on the left of each row). EC apoptosis was studied in the presence (control MO) or in the absence (sih MO) of flow by whole-mount active caspase-3 staining (red). Apoptotic ECs (yellow) were monitored at 30 hours post fertilization (hpf). Lateral view, anterior to the left, dorsal up. Scale bar: 50 μm. B, The proportion of apoptotic ECs (number of apoptotic ECs divided by the total number of ECs) normalized to sih MO-injected embryos was calculated, and mean values are shown with SEM. n≥15 from 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001 using 1-way ANOVA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ubs1Tg control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-angptl4 control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-angptl4 + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-cdh13 control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-cdh13 + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-pdcd2l control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-pdcd2l + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-ptgis control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-ptgis + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnip1 control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnip1 + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnnt2a + MO2-cd74a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnnt2a + MO2-cd74b control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnnt2a + MO2-perp control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnnt2a + MO4-jun control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO2-cd74a control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO2-cd74b control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO2-perp control Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO4-jun control Prim-15 endothelial cell nucleus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-casp3 IHC ubs1Tg control Prim-15 endothelial cell
IHC ubs1Tg + MO1-angptl4 control Prim-15 endothelial cell
IHC ubs1Tg + MO1-angptl4 + MO1-tnnt2a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-cdh13 control Prim-15 endothelial cell
IHC ubs1Tg + MO1-cdh13 + MO1-tnnt2a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-pdcd2l control Prim-15 endothelial cell
IHC ubs1Tg + MO1-pdcd2l + MO1-tnnt2a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-ptgis control Prim-15 endothelial cell
IHC ubs1Tg + MO1-ptgis + MO1-tnnt2a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnip1 control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnip1 + MO1-tnnt2a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnnt2a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnnt2a + MO2-cd74a control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnnt2a + MO2-cd74b control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnnt2a + MO2-perp control Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnnt2a + MO4-jun control Prim-15 endothelial cell
IHC ubs1Tg + MO2-cd74a control Prim-15 endothelial cell
IHC ubs1Tg + MO2-cd74b control Prim-15 endothelial cell
IHC ubs1Tg + MO2-perp control Prim-15 endothelial cell
IHC ubs1Tg + MO4-jun control Prim-15 endothelial cell
Phenotype Details
Fish Conditions Stage Phenotype
ubs1Tg + MO1-angptl4 control Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-angptl4 + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-cdh13 + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, exacerbated
ubs1Tg + MO1-pdcd2l + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, ameliorated
ubs1Tg + MO1-ptgis + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-tnip1 + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-tnnt2a + MO2-cd74a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-tnnt2a + MO2-cd74b control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-tnnt2a + MO2-perp control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, ameliorated
ubs1Tg + MO1-tnnt2a + MO4-jun control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Arteriosclerosis, Thrombosis, and Vascular Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Arterio., Thromb., and Vas. Bio.