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ZFIN ID: ZDB-FIG-170213-1
Serbanovic-Canic et al., 2017 - Zebrafish Model for Functional Screening of Flow-Responsive Genes. Arteriosclerosis, Thrombosis, and Vascular Biology   37(1):130-143 Full text @ Arterio., Thromb., and Vas. Bio.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 1

Flow cessation induces endothelial cell (EC) apoptosis in zebrafish embryos. A, Whole-mount active caspase-3 (red) staining of 30 hours post fertilization (hpf) flk1:EGFP-NLS zebrafish embryos (green EC nuclei) in the presence (control) or absence of flow (sih morpholino oligonucleotide [MO], tricaine). The region outlined with the white box is shown in higher magnification in B; white arrows indicate apoptotic ECs (yellow). C, Zebrafish embryo at 30 hpf. The region outlined with blue box represents the region that is studied in A. The percentage of EC apoptosis (D) and EC numbers (E) in sih MO-injected and tricaine-treated embryos compared with controls was quantified, and mean values are shown with SD; n≥15 from 3 independent experiments, **P<0.01 using 1-way ANOVA. AC, Lateral view, anterior to the left, dorsal up. Scale bars, 50 μm (A), 15 μm (B), and 500 μm (C).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ubs1Tg control Prim-15 endothelial cell nucleus IHC
ubs1Tg chemical treatment: tricaine Prim-15 endothelial cell nucleus IHC
ubs1Tg + MO1-tnnt2a control Prim-15 endothelial cell nucleus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-casp3 IHC ubs1Tg control Prim-15 endothelial cell
IHC ubs1Tg chemical treatment: tricaine Prim-15 endothelial cell
IHC ubs1Tg + MO1-tnnt2a control Prim-15 endothelial cell
Phenotype Details
Fish Conditions Stage Phenotype
ubs1Tg chemical treatment: tricaine Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
ubs1Tg + MO1-tnnt2a control Prim-15 blood circulation decreased occurrence, abnormal
Prim-15 endothelial cell apoptotic process increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Arteriosclerosis, Thrombosis, and Vascular Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Arterio., Thromb., and Vas. Bio.