ZFIN ID: ZDB-FIG-161229-16
Venero Galanternik M. et al., 2016 - Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously. Developmental Biology   419(2):321-335 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 4

Dkk1b is able to inhibit Wnt/β-catenin and Fgf signaling in the absence of glypican4. (A-D) Expression of the Wnt/β-catenin target lef1 in non-transgenic siblings (A) and glypican4fr6 mutants (B) in the leading region of the primordium. (C-D) After heat shock, 28–30 hpf transgenic siblings (C) and glypican4fr6 mutants (D) lose expression of lef1 indicating that Dkk1b is functional and inhibits Wnt target genes. (E-H) The Fgf target pea3 is expressed in the trailing region of the primordium in non-transgenic control siblings (E) and glypican4fr6 mutants (F) and is downregulated after induction of Dkk1-GFP in both backgrounds indicating that the inhibition of Wnt targets results in the loss of Fgf signaling (G-H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 WT control Prim-5 posterior lateral line primordium ISH
gpc4fr6/fr6 control Prim-5 posterior lateral line primordium ISH
gpc4fr6/fr6 ; w32Tg heat shock Prim-5 posterior lateral line primordium ISH
w32Tg heat shock Prim-5 posterior lateral line primordium ISH
lef1 WT control Prim-5 posterior lateral line primordium ISH
gpc4fr6/fr6 control Prim-5 posterior lateral line primordium ISH
gpc4fr6/fr6 ; w32Tg heat shock Prim-5 posterior lateral line primordium ISH
w32Tg heat shock Prim-5 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4fr6/fr6 ; w32Tg heat shock Prim-5 posterior lateral line primordium etv4 expression decreased amount, abnormal
Prim-5 posterior lateral line primordium lef1 expression decreased amount, abnormal
w32Tg heat shock Prim-5 posterior lateral line primordium etv4 expression decreased amount, abnormal
Prim-5 posterior lateral line primordium lef1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 419(2), Venero Galanternik M., Lush, M.E., Piotrowski, T., Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously, 321-335, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.