ZFIN ID: ZDB-FIG-161220-3
Iwanami et al., 2016 - Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development. Cell Reports   17:2259-2270 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 2

Tissue-Restricted Abnormalities in gemin5 Mutants, Related to Figure S5

(A) Normal numbers and distribution of fluorescent lymphoid progenitor cells visualized in an ikaros:eGFP transgenic background (arrows). Scale bar, 10 μm.

(B) Reduced numbers of ikaros-expressing lymphocyte progenitors in the mutant thymus. Scale bar, 25 μm.

(C) Diagnostic whole-mount RNA in situ hybridization pattern in gemin5 mutants using rag1 (thymus encircled in purple), and gh (hypophysis encircled in orange) at 5 dpf. Scale bar, 100 μm.

(D) Lack of thymocytes in the thymus (red circle) at 8 dpf (H&E staining). Scale bar, 25 μm.

(E) Expression of the thymic epithelial marker foxn1 (thymus encircled in purple) at 5 dpf. Scale bar, 100 μm.

(F) Normal receptive capacity of mutant thymic epithelium for whole kidney marrow hematopoietic cells isolated from adult wild-type ikaros:eGFP transgenic fish. At 5 dpf, the number of transplanted cells per embryo was determined. The overall success rate of the transplantation procedure is identical between the genotypes.

(G) Distribution of ikaros-expressing neurons in the mutant hindbrain. Scale bar, 100 μm.

(H) Retinal cell layers (1, ganglion cell layer; 2, inner plexiform layer; 3, inner nuclear layer; 4, outer plexiform layer; 5, outer nuclear layer; 6, photoreceptor cell layer; 7, retinal pigment epithelium) (right panel); H&E staining. Scale bar, 25 μm.

(I) Body size at 8 dpf. Scale bar, 500 μm.

No differences were observed for gemin5+/+ and gemin5+/− fish for any of the analyzed parameters.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fr101Tg standard conditions Prim-5 hematopoietic system IFL
Day 5 common lymphoid progenitor IFL
Day 5 hindbrain neuron IFL
Day 5 thymus IFL
gemin5t26393/t26393; fr101Tg standard conditions Prim-5 hematopoietic system IFL
Day 5 common lymphoid progenitor IFL
Day 5 hindbrain neuron IFL
Day 5 thymus IFL
foxn1 WT standard conditions Day 5 thymic epithelium ISH
gemin5t26393/t26393 standard conditions Day 5 thymic epithelium ISH
gh1 WT standard conditions Day 5 hypophysis ISH
gemin5t26393/t26393 standard conditions Day 5 hypophysis ISH
rag1 WT standard conditions Day 5 thymus ISH
Day 5 thymus T cell ISH
gemin5t26393/t26393 standard conditions Day 5 thymus ISH
Day 5 thymus T cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gemin5t26393/t26393 standard conditions Day 5 T cell differentiation decreased occurrence, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
Days 7-13 thymus T cell absent, abnormal
gemin5t26393/t26393; fr101Tg standard conditions Day 5 thymus EGFP expression decreased amount, abnormal
Day 5 thymus lymphoid progenitor cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.