ZFIN ID: ZDB-FIG-161220-2
Iwanami et al., 2016 - Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development. Cell Reports   17:2259-2270 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

Impaired Early T Cell Development in Zebrafish Mutant for Genes Encoding Components of snRNPs, Related to Figures S1–S3

(A) Diagnostic whole-mount RNA in situ hybridization pattern in lsm8 mutants using rag1 (thymus encircled in purple), and gh (hypophysis encircled in orange) at 5 dpf. Scale bar, 10 μm.

(B–D) Quantification of the rag1/gh ratios as a measure of thymopoietic activity at 5 dpf. The number (n) of individual fish per genotype is indicated; mean ± SEM.

(E) In homozygous lsm8 mutants (additionally transgenic for an ikaros:eGFP reporter), the number of ikaros-expressing cells in the thymus (encircled with dotted lines in middle and right panels) is drastically reduced at 5 dpf. Note the normal numbers of ikaros-positive cells in general hematopoietic tissues at 1 dpf. Scale bars, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fr101Tg standard conditions Prim-5 hematopoietic system IFL
Protruding-mouth common lymphoid progenitor IFL
Protruding-mouth thymus IFL
Day 5 common lymphoid progenitor IFL
Day 5 thymus IFL
lsm8fr100/fr100; fr101Tg standard conditions Prim-5 hematopoietic system IFL
Protruding-mouth common lymphoid progenitor IFL
Protruding-mouth thymus IFL
Day 5 common lymphoid progenitor IFL
Day 5 thymus IFL
gh1 WT standard conditions Day 5 hypophysis ISH
lsm8fr100/fr100 standard conditions Day 5 hypophysis ISH
rag1 WT standard conditions Day 5 thymus ISH
Day 5 thymus T cell ISH
gemin5t26393/t26393 standard conditions Day 5 thymus ISH
lsm8fr100/fr100 standard conditions Day 5 thymus ISH
snapc3t26426/t26426 standard conditions Day 5 thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gemin5t26393/t26393 standard conditions Day 5 T cell differentiation decreased occurrence, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
lsm8fr100/fr100 standard conditions Day 5 T cell differentiation decreased occurrence, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
lsm8fr100/fr100; fr101Tg standard conditions Day 5 thymus EGFP expression decreased amount, abnormal
Day 5 thymus lymphoid progenitor cell decreased amount, abnormal
snapc3t26426/t26426 standard conditions Day 5 T cell differentiation decreased occurrence, abnormal
Day 5 thymus rag1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.