FIGURE

Fig. 1

ID

ZDB-FIG-161220-2

Publication

Iwanami et al., 2016 - Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development

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Fig. 1

Impaired Early T Cell Development in Zebrafish Mutant for Genes Encoding Components of snRNPs, Related to Figures S1–S3

(A) Diagnostic whole-mount RNA in situ hybridization pattern in lsm8 mutants using rag1 (thymus encircled in purple), and gh (hypophysis encircled in orange) at 5 dpf. Scale bar, 10 μm.

(B–D) Quantification of the rag1/gh ratios as a measure of thymopoietic activity at 5 dpf. The number (n) of individual fish per genotype is indicated; mean ± SEM.

(E) In homozygous lsm8 mutants (additionally transgenic for an ikaros:eGFP reporter), the number of ikaros-expressing cells in the thymus (encircled with dotted lines in middle and right panels) is drastically reduced at 5 dpf. Note the normal numbers of ikaros-positive cells in general hematopoietic tissues at 1 dpf. Scale bars, 100 μm.

Expression Data

Genes:	EGFP gh1 rag1
Fish:	WT gemin5^{t26393/t26393} lsm8^fr100/fr100 snapc3^{t26426/t26426} fr101Tg lsm8^fr100/fr100; fr101Tg
Anatomical Terms:	common lymphoid progenitor hematopoietic system hypophysis thymus thymus T cell
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gemin5^{t26393/t26393} lsm8^fr100/fr100 snapc3^{t26426/t26426} lsm8^fr100/fr100; fr101Tg
Observed In:	T cell differentiation thymus thymus lymphoid progenitor cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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