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Fig. 1

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ZDB-IMAGE-161220-2
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Figures for Iwanami et al., 2016
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Fig. 1

Impaired Early T Cell Development in Zebrafish Mutant for Genes Encoding Components of snRNPs, Related to Figures S1–S3

(A) Diagnostic whole-mount RNA in situ hybridization pattern in lsm8 mutants using rag1 (thymus encircled in purple), and gh (hypophysis encircled in orange) at 5 dpf. Scale bar, 10 μm.

(B–D) Quantification of the rag1/gh ratios as a measure of thymopoietic activity at 5 dpf. The number (n) of individual fish per genotype is indicated; mean ± SEM.

(E) In homozygous lsm8 mutants (additionally transgenic for an ikaros:eGFP reporter), the number of ikaros-expressing cells in the thymus (encircled with dotted lines in middle and right panels) is drastically reduced at 5 dpf. Note the normal numbers of ikaros-positive cells in general hematopoietic tissues at 1 dpf. Scale bars, 100 μm.

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