ZFIN Image: Randlett et al., 2013, Fig. S2

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Figure Caption

Fig. S2

Elimination of ACs through Ptf1a Disruption, Related to Figure 2

(A) The ptf1a^sa126 mutation is an A to T conversion, which results in a non-sense mutation at amino acid 126 of 265. This results in a truncation within the loop of the basic helix-loop-helix DNA binding domain.

(B and C) Immunostaining of retinal cryosections using (B) 5E11 to stain AC neurites and (C) anti-HuC/D to stain RGC and AC cell bodies, indicates that some ACs remain in homozygous ptf1a^−/− mutants. Injection of ptf1a morpholinos into ptf1a^−/− mutants, causes a further reduction in ACs numbers, where large stretches of retina lack 5E11 staining and HuC/D positive cells in the AC layer (higher magnification inserts given in: i, and ii).

(D) WT retina with the vsx1GFP labeling BC axons in the IPL (arrow) and ACs/RGCs labeled with HuC/D.

(E) In the ath5^−/−;ptf1a^−/−; ptf1aMO, AC/RGC-free retina, the IPL is positioned along the basal surface of the retina (arrow). Few remaining ACs shown with arrowheads.

Acknowledgments

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