FIGURE

Fig. 6

ID

ZDB-FIG-161202-14

Publication

Bernut et al., 2016 - Mycobacterium abscessus-Induced Granuloma Formation Is Strictly Dependent on TNF Signaling and Neutrophil Trafficking

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Fig. 6

TNF signaling is required for neutrophil recruitment.

(A-D) WT or tnfr morphant Tg(mpx:eGFP) larvae were injected in the otic cavity (A-B) or hindbrain (C-D), with both variants of Mabs (tdTomato, ≈100 CFU, two or three experiments) and imaged at 3 hpi. Representative images (A and C) and number (B and D) (horizontal lines indicate the mean values) of neutrophils at the infection site. Scale bars, 100 μm. (E and F) Confocal image of neutrophils surrounding a 3 dpi R-cord (E) (Scale bars, 50 μm) or a 5 dpi R-abscess (F) (Scale bars, 100 μm) in iv infected WT or tnfr morphant Tg(mpx:eGFP) embryos. Statistical significance was determined by one-tailed Student’s t test (B and D). See also S2–S5 Movies.

Expression Data

Gene:	GFP
Fish:	i114Tg i114Tg + MO3-tnfrsf1a
Condition:	bacterial treatment by injection: Mycobacteroides abscessus
Knockdown Reagent:	MO3-tnfrsf1a
Anatomical Term:	neutrophil
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i114Tg + MO3-tnfrsf1a
Condition:	bacterial treatment by injection: Mycobacteroides abscessus
Knockdown Reagent:	MO3-tnfrsf1a
Observed In:	neutrophil immune response neutrophil response to bacterium neutrophil chemotaxis
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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