FIGURE

Fig. S2

ID

ZDB-FIG-161202-17

Publication

Bernut et al., 2016 - Mycobacterium abscessus-Induced Granuloma Formation Is Strictly Dependent on TNF Signaling and Neutrophil Trafficking

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Fig. S2

Early TNF-? Response Induction following Mabs infections.

(A) Tg(tnf-?:eGFP-F) embryos were infected into caudal vein with ?100 Mabs-expressing tdTomato. Microscopy showing the representative expression of tnf-? close to the injection site at 2 hpi. Scale bar, 300 ?m. (B-C) Fluorescence microscopy analysis of the GFP expression in Tg(tnf-?:eGFP-F/mpeg1:mCherryF) (B) or Tg(tnf-?:eGFP-F/LysC:DsRed) (C) double transgenic embryos at 1 day following intravenous infection by either ?100 R- or S-expressing E2-Crimson. (B) Close-up to the tip of the tail revealing that the transcriptomic tnf-? expression is detected in infected macrophages or in macrophages close to the infected tissue. Scale bars, 200 ?m. (C) Confocal images of a single Mabs-infected macrophage (yellow arrow) close to the injection site induced a strong tnf-? expression. Neutrophils are indicated with white arrows. Scale bar, 10 ?m. (D) tnf-? expression was tested in Tg(tnf-?:GFP-F) zebrafish embryo in absence of macrophages (lipo-clodronate injection). Embryos were infected with Mabs (R variant, ?100 CFU) in the otic cavity and the proportion of infected embryos with eGFP-positive cells at 2 hpi counted. Graphs represent the mean value of two independent experiments (n = 10).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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