ZFIN ID: ZDB-FIG-161027-36
Middel et al., 2016 - Dysferlin-mediated phosphatidylserine sorting engages macrophages in sarcolemma repair. Nature communications   7:12875 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 1

Macrophages remove repair patch.

(a-c) Repair patch (AnxA2a-mO, a, white arrow) and macrophages (mpeg1:GFP, b, white arrows) before (Initial), 240 s and 1,500 s after wounding. A macrophage ingests parts of repair patch (green arrows). (c) Merged views of a,b. Note the bleed-through of AnxA2a-mO into the GFP channel (b). (d) Repair patch removal in control (left, n=37) and macrophage-depleted embryos (KD, right, n=25, Fisher’s exact test P<10-8) over 20 h. (e) Crispr/Cas9 knock-out of irf8 impairs repair patch removal. In controls, the repair patch was removed in 32% of injured myofibers (n=19) within one hour after injury. In the irf8-KO embryos the patch was present in all cases of injured myofibers examined (n=15). This suggests that morpholino triple knock-down is inefficient and the same effect on macrophages can be achieved by elimination of irf8 at the stage analysed. Significance was checked with Fishers exact test at P<0.05 (P=0.023895). Scale bar, 4 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gl22Tg light damage: sarcolemma: muscle cell Protruding-mouth to Day 5 macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl22Tg + MO1-irf8 + MO1-spi1b + MO3-csf3r light damage: sarcolemma: muscle cell Protruding-mouth - Day 5 macrophage absent, abnormal
Protruding-mouth - Day 5 muscle cell cell wall repair process quality, abnormal
irf8ka90/ka90 ; gl22Tg light damage: sarcolemma: muscle cell Protruding-mouth - Day 5 macrophage absent, abnormal
Protruding-mouth - Day 5 muscle cell cell wall repair process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.