FIGURE

Fig. 1

ID
ZDB-FIG-160725-1
Publication
Prabhudesai et al., 2016 - LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation
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Fig. 1

LRRK2 knockdown by translational MO produces axis curvature defects, ocular abnormalities, and edema. A: Western blot analysis showing LRRK2 expression in wt embryos and in LRRK2 morphant embryos using a LRRK2-specific antibody normalized to GAPDH. B: Quantification of LRRK2 expression normalized to GAPDH. Y-axis shows LRRK2 expression in LRRK2 morphant embryos as a percentage of LRRK2 expression in wt embryos. n = 3 experiments for each group; 12 embryos in each group in each experiment. C: Lateral view of a 3-dpf LRRK2 morphant embryo with axis curvature defects (asterisk). Lateral view of a 3-dpf LRRK2 5-base mismatch MO-injected embryo (D) and a 3-dpf wt embryo both showing a wt phenotype and straight tail (E). F: Dorsal view of a 3-dpf LRRK2 morphant embryo with axis curvature defects (asterisk) and with edema in otic vesicles (arrow in main image) and lens (arrow in inset). Dorsal view of a 3-dpf LRRK2 5-base mismatch MO-injected embryo (G) and a 3-dpf wt embryo both with normal lenses, otic vesicles, and a straight tail (H). I: Lateral view of a 6-dpf LRRK2 morphant embryo showing edema around the eyes and ventral curvature defects (bent tail; pound sign). Lateral view of a 6-dpf LRRK2 5-base mismatch MO-injected embryo (J) and a 6-dpf wt embryo both showing normal eye development and a straight tail (K). L: Dorsal view of a 6-dpf LRRK2 morphant showing distinct edema around the eyes (arrows). Dorsal view of a LRRK2 5-base mismatch MO-injected embryo (M) and a 6-dpf wt embryo both showing normal eyes (N). O: Ventral view of 3-dpf LRRK2 morphant showing coloboma in one of the eyes (arrow). P: Ventral view of a LRRK2 5-base mismatch MO-injected embryo showing normal eyes. n = 2,932 for LRRK2 translational morphants, n = 2,100 for LRRK2 5-base mismatch controls MO, n = 2,142 for wt. ***P < 0.001 (Student′s t-test). Scale bars = 0.5 mm.

Expression Data
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci. Res.